Part:BBa_K658016:Design
LuxI->LuxR->lux pR->GFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1874
Illegal BsaI.rc site found at 2601
Design Notes
Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device.
The device is constructed by combining two parts.
The two parts are proven to be right.
From the electrophoretogram,we can see that the part is successfully constructed.
Source
K658012
References
[1]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745.
[2]Devine J.H,Shadel G.S,Baldwin T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proceedings of the National Academy of Sciences.1989.86(15):5688-5692.
[3] http://www.uniprot.org/uniprot/P12747
[4]Miki T,Yoshioka K,Horiuchi T. Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. Molecular Biology,1984,177(4):605-625.
[5] http://en.wikipedia.org/wiki/N-Acyl_homoserine_lactone
[6] Hanzelka BL, Greenberg E. Quorum sensing in Vibrio fischeri: evidence that S-adenosyl methionine is the amino acid substrate for autoinducer synthesis[J]. Journal of Bacteriology, 1996, 178(17): 5291-5294.
[7] Urbanowski M, Lostroh C, Greenberg E. Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein[J]. Journal of Bacteriology, 2004, 186(3): 631-637.
[8] Hansen LH, Knudsen S, Sorensen SJ . The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Curr microbiol，1998， 36 (6): 341–345.
[9]Bernard P，Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. Molecular Biology,1992,226:735-745.